Isolation of germination mutants of Dictyostelium discoideum.
نویسندگان
چکیده
A simple method to separate spores from amoebae of Dictyostelium discoideum has been devized and used to isolate spore germination mutants. A subclass of these mutants is temperature sensitive for germination and growth.
منابع مشابه
Gamma-ray-induced spore germination of Dictyostelium discoideum.
(60)Co gamma rays can induce germination of the spores of the cellular slime mold, Dictyostelium discoideum, in the absence of heat shock, amino acids, or bacteria food source. About 65% amoebae emergence occurs by 13 hr after a dose of 180 krad.
متن کاملSpore germination promoter of Dictyostelium discoideum excreted by Aerobacter aerogenes.
The nutrient medium in which Aerobacter aerogenes was grown, contains a spore germination promoter (SGP) for the cellular slime mould Dictyostelium discoideum. SGP can cuase synchronous spore germination in a short time, and triggers the germination process in just a few minutes. Germination-promoting capacity of SGP decreases as it comes in contact with increasing number of spores. When spores...
متن کاملIsolation and characterization of mutations affecting UDPG pyrophosphorylase activity in Dictyostelium discoideum.
Dimond, R. L., Loomis, W. F. "Acetylglucosaminidase mutants in Dictyostelium discoideum." 1973 Genetics. 74: 64 Cardelli, J. A., Dimond, R. L. "Effect of Starvation and Cell-Cell Interaction on Protein Synthesis in Dictyostelium-discoideum." The 20th Annual Meeting of the American Society for Cell Biology, Cincinnati, Ohio, USA, 1980, J. Cell Biology, 87: (2 Part 2) 275A Knecht, D. A., Dimond, ...
متن کاملCorrelation between acrasins and spore germination inhibitors in cellular slime molds.
Discadenine,3-(3-amino-3-carboxypropyl)-N6-delta 2-isopentenyladenine, which inhibits spore germination, was previously found in Dictyostelium discoideum. Studies on the distribution of discadenine in different species of cellular slime molds by high-pressure liquid chromatography showed that discadenine is present in D. discoideum, Dictyostelium purpureum, and Dictyostelium mucoroides, but not...
متن کاملA Dictyostelium discoideum cellulase is a member of a spore germination-specific gene family.
A member of the 270 spore germination-specific gene family in Dictyostelium discoideum is shown to encode a cellulase (endo-(1,4)-beta-D-glucanase, EC 3.2.1.4) activity. The 270-6 deduced protein shows 38% identity and 58% similarity to an avocado (Persea americana) cellulase. During spore germination in Dictyostelium extracellular cellulase activity starts to accumulate coincident with the app...
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 117 2 شماره
صفحات -
تاریخ انتشار 1974